Quick-DNA/RNA Pathogen Miniprep

Pathogen DNA/RNA isolation from vectors, tissue and biological samples.

Highlights


  • Spin-column purification of pathogen (virus, bacteria, protozoa) DNA/RNA from a wide variety of vectors (mosquitoes, fleas, ticks, etc.) and tissue types (mammals, birds, etc.) stored in DNA/RNA Shield™.
  • DNA/RNA is ready for any sensitive downstream applications (e.g., Next-Gen sequencing, RT/PCR, etc.)


Description


Quick-DNA/RNA Pathogen Miniprep kit is a spin-column based purification of pathogen (virus, bacteria, protozoa) DNA and RNA from a wide variety of vectors (mosquitoes, fleas, ticks, etc.) and tissue types (mammals, birds, etc.) collected, transported and stored in DNA/RNA Shield™. The kit features a storage/lysis buffer system and can be combined with high density ZR BashingBead™ Lysis Tubes (optional) to facilitate complete homogenization of hard-to-lyse samples for efficient nucleic acid isolation. Small (>50 nt) and large (>200 kb) DNA and RNA are bound to Zymo-Spin™ IIC Columns, washed and then eluted. The isolated high-quality nucleic acids are suitable for all downstream applications such as Next-Gen sequencing, hybridization-based and RT/PCR detection.

Technical Specifications


Equipment Microcentrifuge
Purity DNA/RNA is ready for any sensitive downstream applications (e.g., Next-Gen sequencing, RT/PCR, etc.)
Sample Source Vectors (mosquitoes, fleas, ticks, other tough-to-lyse insects) and tissue types (animal tissue, plants, other hosts) processed and stored in DNA/RNA Shield™.
Size Range 50 nt to ~200 kb
Yield 50 µg DNA/RNA binding capacity (spin-column), ≥25 µl elution volume

Citations


Kit Components


Quick-DNA/RNA Pathogen Miniprep

R1042/R1043

Cat # Name Size
R1042 Quick-DNA/RNA Pathogen Miniprep 50 Preps
R1043 Quick-DNA/RNA Pathogen Miniprep 200 Preps

Documents


Protocol: 
Datasheet: 
SDS (MSDS):