Highlights

Unbiased Lysis: Efficient and unbiased lysis of microbes including gram positive/negative bacteria, fungi, protozoans, and viruses from any sample including feces, soil, plant, water, biofilms, swabs, saliva, body fluids, etc.
Ultra-Pure: High-quality DNA/RNA (including mall/microRNAs) are inhibitor-free and ready for RT/qPCR and microbiome measurements using Next-Gen sequencing.
High Sensitivity: Increased detection limit of very low abundance organisms.

Description

The ZymoBIOMICS MagBead DNA/RNA kit provides a high-throughput, magnetic bead-based purification of both high-quality DNA and total RNA (including small/microRNAs) from the same starting sample. The provided DNA/RNA Shield inactivates infectious agents and is ideal for sample storage at ambient temperatures. The extraction method utilizes magnetic beads for DNA/RNA extraction without the use of phenol and is eluted into =50 µl of ZymoBIOMICS DNase/RNase-Free Water. DNA/RNA is ready for any downstream application including Next-Gen Sequencing, RT-qPCR,, etc.

Technical Specifications

Binding Capacity	15 µg DNA/RNA per 30 µl ZymoBIOMICS MagBinding Beads.
Equipment Needed	Magnetic stand or separator, heat block, liquid handler or robotic sample processer (user provided).
Purity	High-quality RNA is ready for Next-Gen Sequencing, RT/PCR, hybridization, etc.
Recommended Materials	(sold separately) – 96-well Collection Plate (C2002; capacity is up to 1.2 ml/well), 96-Well Block (P1001; capacity is up to 2 ml/well), 96-well Elution Plate (C2003), Cover Foil (C2007), ZR-96 MagStand (P1005).
Sample Preservation	DNA/RNA Shield lyses cells, inactivates nucleases and infectious agents and is ideal for safe sample storage and transport at ambient temperatures.
Sample Types	Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA and RNA are efficiently isolated from = 50 mg of mammalian feces, = 50 mg soil, and 5-20 mg (wet weight) of fungal bacterial cells, biofilms, water, and swabs.
Size Limits	Capable of recovering DNA and total RNA ≥ 17 nucleotides.
Storage	DNA and RNA eluted with ZymoBIOMICS DNase/RNase-Free Water (provided)can be stored frozen. The addition of RNase inhibitors is highly recommended for prolonged storage.

Citations

Kit Components

Cat #	Name	Size
R1100-50	DNA/RNA Shield	50 ml
R1100-250	DNA/RNA Shield	250 ml
R1101	DNA/RNA Shield-Fecal Collection Tube	10 pack
R1103	DNA/RNA Shield Lysis Tubes (Microbe)	50 pack
R1104	DNA/RNA Shield Lysis Tubes w/Swab (Microbe)	50 pack
R1106	DNA/RNA Shield Collection Tube w/Swab (1 ml fill)	10 pack
R1107	DNA/RNA Shield Collection Tube w/Swab (1 ml fill)	50 pack
D3001-2-20	Proteinase K w/ Storage Buffer Set	20 mg
D3001-2-5	Proteinase K w/ Storage Buffer Set	5 mg
D4302-5-1	ZymoBIOMICS DNase/RNase Free Water	1 ml
D4302-5-10	ZymoBIOMICS DNase/RNase Free Water	10 ml
D4302-5-30	ZymoBIOMICS DNase/RNase Free Water	30 ml
D4302-5-50	ZymoBIOMICS DNase/RNase Free Water	50 ml
D4302-6-12	ZymoBIOMICS MagBinding Beads	12 ml
D4302-6-6	ZymoBIOMICS MagBinding Beads	6 ml
D7001-1-200	DNA/RNA Lysis Buffer	200 ml
D7001-1-50	DNA/RNA Lysis Buffer	50 ml
D7010-2-200	DNA/RNA Prep Buffer	200 ml
D7010-2-50	DNA/RNA Prep Buffer	50 ml
E1010	DNase I Set	250 U
R2130-1-120	MagBead DNA/RNA Wash 1	120 ml
R2130-1-30	MagBead DNA/RNA Wash 1	30 ml
R2130-2-20	MagBead DNA/RNA Wash 2	20 ml
R2130-2-80	MagBead DNA/RNA Wash 2	80 ml
S6002-96-3	ZR-96 BashingBead Lysis Rack	0.5 mm & 0.1 mm
S6012-50	ZR BashingBead Lysis Tubes (0.1 & 0.5 mm)	50 Tubes
R1102	DNA/RNA Shield Collection Tube	50 pack

ZymoBIOMICS MagBead DNA/RNA

R2135/R2136

Cat #	Name	Size
R2135	ZymoBIOMICS MagBead DNA/RNA	1 x 96 Preps
R2136	ZymoBIOMICS MagBead DNA/RNA	4 x 96 Preps

Documents

Protocol:

Datasheet:

SDS (MSDS):