ELISA Kit for Neuropeptide FF (NPFF)

product no

CEG003Ra

itme name

Neuropeptide FF

formate

48T, 96T, 96Tx5, 96Tx10, 96Tx100

detection range

12.5-200pg/mL

organism species

Rattus norvegicus (Rat)

sensitivity

The minimum detectable dose of this kit is typically less than 4.7pg/mL

sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

assay length

2h

method

Competitive Inhibition

  • Linearity
  • Precision
  • Stability
  • Assay Procedure Summary
  • Alternative Names
  • Recovery
  • Specificity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neuropeptide FF (NPFF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuropeptide FF (NPFF) were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuropeptide FF (NPFF) were tested on 3 different plates, 8 replicates in each plate.

CV(%) = SD/meanX100

Intra-Assay: CV<10%

Inter-Assay: CV<12%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.

To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

1. Prepare all reagents, samples and standards;

2. Add 50µL standard or sample to each well.

    And then add 50µL prepared Detection Reagent A immediately.

    Shake and mix. Incubate 1 hour at 37°C;

3. Aspirate and wash 3 times;

4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;

5. Aspirate and wash 5 times;

6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;

7. Add 50µL Stop Solution. Read at 450 nm immediately.
FMRFAL; Neuropeptide FF-Amide Peptide Precursor; Pro-FMRFamide-related neuropeptide FF
Matrices listed below were spiked with certain level of recombinant Neuropeptide FF (NPFF) and the recovery rates were calculated by comparing the measured value to the expected amount of Neuropeptide FF (NPFF) in samples.


This assay has high sensitivity and excellent specificity for detection of Neuropeptide FF (NPFF).

No significant cross-reactivity or interference between Neuropeptide FF (NPFF) and analogues was observed.

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