Nucleic Acid Purification

DNA and RNA need to be isolated from their respective hosts- eukaryotic cells, bacteria, plasmid, virus and yeast, for downstream applications such as blotting, PCR, cloning and sequencing. Most clinical and research laboratories require some kind of nucleic acid processing for Research & Diagnostics . High throughput labs can take advantage of automated systems to streamline workflow and reduce repetitive stress. Choice of purification system will depend on volume, number of samples, desired turnaround time and state of the nucleic acid- fragment, chromosome, mRNA, super coiled DNA etc. Purification is usually the first step in a long series of experimentation, quality is the goal.