Control for bisulfite conversion; DNA methylation quantitation

Highlights

Purified, non-methylated and methylated human DNA for use as negative and positive controls in methylation detection applications. Standards can be assayed in parallel with samples to monitor bisulfite conversion efficiency.
Ideal controls for bisulfite sequencing PCR (BSP) and methylation specific PCR (MSP).
Each standard is provided with a primer set to amplify a fragment of DNA after bisulfite conversion

Description

The Human Methylated & Non-methylated DNA Set consists of two DNA controls (a CpG-methylated human DNA standard and a non-methylated human DNA standard), with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation family of products. This DNA set can be included as a positive and negative control to assess the efficiency of bisulfite conversion of DNA. The non-methylated human DNA is purified from the HCT116 DKO cell line, which contains genetic knockouts of both DNA methyltransferases DNMT1 (-/-) and DNMT3b (-/-). The methylated DNA standard is purified HCT116 DKO DNA that has been enzymatically methylated at CpG sites.

Technical Specifications

Concentration	250 ng/µl
Format	Purified genomic DNA
Storage	≤ -20°C

Citations

Human Methylated & Non-methylated DNA Set

D5014 / D5014-1 / D5014-2

Cat #	Name	Size
D5014	Human Methylated & Non-methylated DNA Set	5 µg/20 µl
D5014-1	Human HCT116 DKO Non-Methylated DNA	5 µg/20 µl
D5014-2	Human HCT116 DKO Methylated DNA	5 µg/20 µl

Documents

Protocol:

Datasheet:

SDS (MSDS):