The Zyppy-96 Plasmid Miniprep is the fastest and simplest high-throughput plasmid DNA kit available for efficient isolation of plasmid DNA from E. coli. This plasmid DNA kit features a modified alkaline lysis system that is pellet-free. This completely bypasses tedious centrifugation, pelleting, and re-suspension steps common to conventional procedures. Instead, the uniquely formulated Deep Blue Lysis Buffer is added directly to the bacterial cultures in a 96-well block. Buffer neutralization and lysate separation steps are assisted with a specially designed Neutralization/Clearing Buffer. Eluted plasmid DNA is high quality, endotoxin-free, and well-suited for use in restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications including transfection.

Applicable For	Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA.
Elution Volume	≥ 30 µl
Equipment	Centrifuge with micro plate carriers
Processing Time	45 min
Purity	Typical Abs 260/280 ≥ 1.8.
Size Range	Up to 25 kb
Yield	Up to 10 µg per preparation depending on the plasmid copy number, culture growth conditions, and strain of E. coli processed.

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