- Validated Unbiased for Microbiome Measurements: Unbiased cellular lysis validated using the ZymoBIOMICS Microbial Community Standard.
- Inhibitor-Free DNA from Any Sample: Isolate ultra-pure DNA ready for any downstream application.
- Certified Low Bioburden: Boost your detection limit for low abundance microbes.
|Applicable For||All sensitive downstream applications such as qPCR and Next-Generation Sequencing.|
|Elution Volume||≥ 20 µl DNA Elution Buffer or DNase free water|
|Equipment||Centrifuge w/ microplate carriers, 96-well plate/block disruptor or pulverizer|
|Processing Volume||Fecal ≤100 mg, Soil ≤ 100 mg, Cells ≤ 20 mg (Approximately equal to 2×108 bacterial, 2×107 yeast, or 2×106 mammalian cells)|
|Purity||High quality, inhibitor-free DNA is eluted with ZymoBIOMICS DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing|
|Sample Source||Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water.|
|Sample Storage||Eluted DNA should be stored at ≤ -20°C.|
|Size Range||Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield.|
|Yield||Up to 5 µg total DNA can be eluted into 20 µl|
ZymoBIOMICS 96 DNA Kit