Highlights

High Purity: Purify endotoxin-free BAC/PAC plasmid DNA (up to ~200 kb) for sequencing, PCR, restriction endonuclease digestion, etc.
Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization
Unique column design: Zero buffer retention and low elution volume (10 µl)

Description

The ZR BAC DNA Miniprep Kit efficiently isolates BAC plasmid DNA or other large plasmids (e.g., PAC) from E. Coli using a procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol with color-coded reagents that allow easy visualization of complete bacterial cell lysis and neutralization. The innovative Zymo-Spin™ IC-XL columns are optimized for high yield, endotoxin-free plasmid DNA recovery. BAC DNA purified using the ZR BAC DNA Miniprep Kit is ideal for sequencing, PCR, endonuclease digestion, etc.

Technical Specifications

Applicable For	Transfection, transformation, sequencing, restriction endonuclease digestion, PCR, in vitro transcription, and other sensitive applications.
Elution Volume	≥ 10 µl
Equipment	Microcentrifuge
Processing Time	15 min
Purity	Typical Abs 260/280 ≥ 1.8 and Abs 260/230 ≥ 2.0
Size Range	Up to ~200 kb
Yield	Up to 10 µg per preparation, depending on the plasmid copy number, initial volume of E. Coli culture processed, and culture growth conditions. The typical yield is 1 µg per 3 ml of culture.

Citations

Kit Components

Cat #	Name	Size
D3004-4-1	DNA Elution Buffer	1 ml
D3004-4-16	DNA Elution Buffer	16 ml
D3004-4-4	DNA Elution Buffer	4 ml
D4027-1-10	Buffer P1 (Red)	10 ml
D4027-1-160	Buffer P1 (Red)	160 ml
D4027-1-20	Buffer P1 (Red)	20 ml
D4027-1-80	Buffer P1 (Red)	80 ml
D4027-2-10	Buffer P2 (Green)	10 ml
D4027-2-160	Buffer P2 (Green)	160 ml
D4027-2-20	Buffer P2 (Green)	20 ml
D4027-2-80	Buffer P2 (Green)	80 ml
D4027-3-12	Buffer P3 (Yellow)	12 ml
D4027-3-220	Buffer P3 (Yellow)	220 ml
D4027-3-440	Buffer P3 (Yellow)	440 ml
D4027-3-50	Buffer P3 (Yellow)	50 ml
D4027-4-12	Plasmid Wash Buffer	12 ml
D4027-4-24	Plasmid Wash Buffer	24 ml
D4027-4-48	Plasmid Wash Buffer	48 ml
D4027-4-6	Plasmid Wash Buffer	6 ml
D4036-3-15	Endo-Wash Buffer	15 ml
D4036-3-30	Endo-Wash Buffer	30 ml
D4036-3-6	Endo-Wash Buffer	6 ml
D4036-3-60	Endo-Wash Buffer	60 ml
C1001-50	Collection Tubes	50 Pack
C1002-25	Zymo-Spin IC-XL	25 Pack
C1002-50	Zymo-Spin IC-XL	50 Pack

ZR BAC DNA Miniprep Kit

D4048/D4049

Cat #	Name	Size
D4048	ZR BAC DNA Miniprep Kit	25 Preps
D4049	ZR BAC DNA Miniprep Kit	100 Preps

Documents

Protocol:

SDS (MSDS):