Highlights
- Quick & Easy: Extract DNA and RNA from any sample including cells, solid tissue, whole blood, biological liquids, FFPE tissue, environmental (plant/seed), swabs (stool, soil, microbial samples), etc.
- Ultra-Pure: Ready for Next-generation sequencing, RT-qPCR, arrays, etc. DNase I included (Plus kit and MagBead only)
Description
Technical Specifications
| Equipment | Microcentrifuge, vortex, and heat block/bath (55°C) |
|---|---|
| Purity | DNA and RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8. |
| Sample Source | Any cells, solid tissue, whole blood, biological fluids, FFPE tissue, environmental (plant/seed), swabs (stool, soil, microbial samples), samples stored in DNA/RNA Shield™, etc. |
| Size Range | Genomic DNA ≥40 kb and Total RNA ≥17 nt |
| Yield | 100 µg DNA and RNA (binding capacity), ≥50 µl (elution volume) |
Product FAQ
Q1: How to purify RNA from samples stored in RNAlater?
It is best to remove the RNAlater as it can interfere with the purification process:
– For cells: Pellet via centrifugation at up to 5,000 x g and remove RNAlater (supernatant)
– For tissue: Remove tissue using forceps, eliminate any excess reagent or crystals (e.g. PBS rinse) and proceed with addition of RNA Lysis Buffer.
If RNAlater cannot be removed, add 1 volume of RNase-free water or PBS (1:1), then add 4 volumes DNA/RNA Lysis Buffer (4:1).
Q2: Is the kit compatible with samples stored in DNA/RNA Shield?
Yes, bring samples homogenized and stored in DNA/RNA Shield to room temperature (20-30ºC). Add 1 volume of DNA/RNA Lysis Buffer (1:1) and mix well. Proceed with DNA Purification.
Q3: Can samples be stored in DNA/RNA Lysis Buffer prior to processing?
Yes, samples in DNA/RNA Lysis Buffer are stable overnight at room temperature and can be stored frozen (-80°C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.
Q4: What is the difference between the Quick-DNA/RNA Miniprep (D7001) and Quick DNA/RNA Miniprep Plus (D7003)?
Use the Quick-DNA/RNA Miniprep for cells and soft tissues.
The “Plus” kit accommodates all sample types (cells, tissue, blood) and comes with DNA/RNA Shield (sample collection, transport, storage at ambient temperature).
Q5: Is DNase I available for individual purchase?
Yes, the catalog number for the DNase I set (DNase and DNA Digestion Buffer) that we offer is E1010.
Q6: Can I use other DNase I enzymes (or sets)? (e.g. Qiagen DNase I)?
The kit is optimized with Zymo’s DNase, however DNase from other manufacturers can be used. Follow the respective protocol for on-column DNase treatment. If the DNase does not have a protocol, proceed with in-tube DNase treatment post extraction, then purification using either “Reaction Clean-up procedure” or RCC-5.
Q7: How to store DNase-I following resuspension?
Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.
Q8: Is Quick-DNA/RNA suitable for very small numbers of cells?
Yes, the Quick-DNA/RNA MicroPrep Plus (#D7005) is designed and capable of purifying RNA down to single cell inputs (picogram amounts). A sensitive quantification method is needed (e.g. Qubit, qPCR, etc.)
Q9: Is the kit compatible with blood samples stored in PAXgene Blood Tubes?
Yes, the kit is compatible with these anticoagulants.
Q10: Is the DNase-I treatment necessary?
If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.
Q11: Will the kit isolate small RNAs?
Yes, this kit will isolate small/micro RNA’s = 17 nucleotides.
Q12: Is it possible to extract proteins with the Quick-DNA/RNA kit?
Yes, proteins can be Acetone Precipitated post RNA binding step. Please request supplementary protocol from Zymo Research Technical Support.
Q13: I ran out of DNA/RNA Wash Buffer. Can I use something else?
Yes. Use 80% ethanol as a substitute. DNA/RNA Wash Buffer is also sold separately.
Q14: Is the DNA and RNA suitable for Next-Gen Sequencing or other sensitive downstream applications?
Yes, both DNA and RNA is high quality (A260/A280 >1.8, A260/A230 >1.8) and suitable for any downstream application, including NGS, qPCR, etc.
Citations
Reviews
“This kit was invaluable. I was able to get at least 1 ug of RNA and several hundred nanograms of DNA from each sample – all of it high quality. Much better than my previous experience with a different dual DNA/RNA isolation kit.”
– Julie P. (CU Anschutz Medical Campus)
“I usually recommend this kit for those who want to extract both DNA and RNA from a single sample. We have even had great quality and yield for single insect samples.”
– Robert B. (Harvard University)
“The kit is very economical for simultaneous extraction of high quality DNA and RNA from very small amount of biological samples. More particularly, RNA is of good quality that suits best for applications like next-generation sequencing (RNA-Seq).”
– Muhammad A. (Northwestern University)
“The kit is awesome! Besides saving a lot of time purifying gDNA and RNA at the same time, it also allows me to use cultures for other purposes since both can be extracted from the same sample.”
– Fernando B. (MIT)
“”Love this kit. I work with small mites and I get great results. I have tried other kits and none perform the same. Technical support is also great and they do their best to answer your questions and concerns.”
– Laura T. (USDA PPQ APHIS CPHIST)
Kit Components
| Cat # | Name | Size |
|---|---|---|
|
R1200-25 |
DNA/RNA Shield (2X Concentrate) | 25 ml |
|
W1001-1 |
DNase/RNase-Free Water | 1 ml |
|
W1001-10 |
DNase/RNase-Free Water | 10 ml |
|
D7001-1-50 |
DNA/RNA Lysis Buffer | 50 ml |
|
D7001-2-10 |
DNA Prep Buffer | 10 ml |
|
D7010-2-50 |
DNA/RNA Prep Buffer | 50 ml |
|
D7010-3-24 |
DNA/RNA Wash Buffer (Concentrate) | 24 ml |
|
R1200-1-5 |
PK Digestion Buffer | 5 ml |
|
C1001-50 |
Collection Tubes | 50 Pack |
|
C1006-50-F |
Spin-Away Filters | 50 Pack |
|
C1006-50-G |
Zymo-Spin IIICG Columns | 50 Pack |
|
E1010-1-4 |
DNA Digestion Buffer | 4 mL |
Quick-DNA/RNA Miniprep Plus Kit
D7003/D7003T
| Cat # | Name | Size |
|---|
