Removes PCR inhibitors such as polyphenolics, humic/fulvic acids, tannins, melanin, etc. from nucleic acid solutions to yield high quality DNA or RNA.
Fast, one-step procedure for cleaning impure samples prior to PCR, sequencing, reverse transcription (RT), etc.
Description
The OneStep PCR Inhibitor Removal Kits are PCR inhibitor clean up kits that contain all the components needed for efficient removal of contaminants that can inhibit downstream enzymatic reactions (e.g. PCR and RT) from DNA and RNA preparations. The column matrices have been specifically designed for the efficient removal of polyphenolic compounds, humic/fulvic acids, tannins, melanin, etc. from the most impure DNA and RNA preparations. Sample clean-up is as simple as applying, spinning, and recovering a sample from the column.
Technical Specifications
Applicable For
Cleaned samples are ready for PCR, sequencing, reverse transcription (RT), etc.
Elution Volume
50 – 200 µl
Equipment
Microcentrifuge
Purity
High quality, enzymatic-reaction ready DNA/RNA products.
Sample Source
DNA/RNA samples contaminated with enzymatic reaction inhibitors.
Sample Storage
Eluted DNA/RNA can be used immediately or stored at ≤ -20°C.
Type
DNA and RNA
Yield
50 – 90% recovery
Product FAQ
Q1: What is the smallest input volume of DNA that can be added to the OneStep Inhibitor Removal kit?
The smallest volume will be 50 ul.
Q2: What is the largest input volume of DNA that can be added to the OneStep Inhibitor Removal kit?
The OneStep PCR Inhibitor Removal Kit can process about 500 µl of sample at a time. However, the same column can be reused for the same sample.
Q3: Can I centrifuge at a lower speed than 3,500 x g for the 96-well kit?
No, the kit requires the speed to be at 3,500 x g. Centrifuging at lower speeds can result in inefficient sample elution.
Citations
Reviews
“The ease. Holy cow, this thing was so freaking simple compared to similar products”
– Christian H. (University of Georgia)
“It was VERY fast and yielded the intended results…PCR products from previously non-amplifiable DNA.”
– Tara N. (United States Agricultural Research Service)
