The DNA Clean & Concentrator-500 (DCC-500) is our highest capacity PCR purification kit of the DNA Clean & Concentrator products. It is designed for the rapid, large format purification and concentration of up to 500 µg of high quality DNA from samples such as large-scale restriction endonuclease digestions and crude DNA preparations. Eluted DNA is well suited for use in PCR, DNA sequencing, DNA transfection, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, etc. The entire DNA purification/concentration procedure typically takes less than 20 minutes.

Detergent Tolerance	≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS
Elution Volume	≥ 2 ml
Equipment	Microcentrifuge and centrifuge or vacuum source.
Purity	Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions.
Sample Source	DNA from large-scale sample sources including restriction endonuclease digestions and “crude” DNA preparations from cell-free lysates.
Size Range	50 bp to 23 kb
Yield	≤ 500 µg total DNA can be recovered. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%.

Q1: What is the minimum input volume of DNA sample?

Q2: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?

Q3: How many times can columns be reloaded?

Q4: How to process naked DNA stored in DNA/RNA Shield?

Q5: What is the lower limit and minimal amount of DNA that can be recovered?

Q6: What to do if ethanol addition to the DNA Wash Buffer was omitted?

“I sampled this kit next to a kit we already use. I had three of the same sample, two for the Zymo kit and one for the other. On one of the ones designated for your kit, I accidentally added the sample directly to the column filter, then added other reagents (I figured I might as well try it). The other I did as per the instructions. The one on which I goofed gave a similar purification and concentration as the other kit, while the one I did properly yielded approximately four times the DNA per µL as the other kit. I am very happy with this result, and when I rerun my samples, I plan to use your product.”

– Rosalind P. (University of Arkansas for Medical Sciences)

“Almost no loss of elution buffer at the final step. It is quite impressive compared to the other kits I have used, which lose about 5-7µl.”

– Joseph R. (Miller School of Medicine, University of Miami)

“It was a very simple procedure and it gave a concentration ten times the original amount.”

– Kimberly M. (University of Pennsylvania)

“The elution volume is low enough to concentrate any sample very well and the efficiency is not compromised at all.”

– Marissa V. (Harvard Medical School)

“This kit performed as described. I was able to obtain good quality and yield of the DNA -better than with a couple of competitor’s kits.”

– Kathleen B. (SUNY-ESF)