- Clean and concentrate up to 5 µg DNA with ≥ 6 µl elution in as little as 2 minutes with 0 µl wash residue carryover.
- Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer.
- Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.
|Detergent Tolerance||≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS|
|Elution Volume||≥ 6 µl|
|Purity||A260/A280 > 1.8|
|Sample Source||DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc.|
|Size Range||50 bp to 23 kb|
|Yield||≤ 5 µg total DNA can be recovered. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%.|
Q1: What is the difference between capped and uncapped?
Q2: What is the minimum input volume of DNA sample?
Q3: How many times can columns be reloaded?
Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?
Q5: How can I process naked DNA stored in DNA/RNA Shield?
Q6: What is the lower limit and minimal amount of DNA that can be recovered?
Q7: What should I do if I did not add ethanol to the DNA Wash Buffer before starting?
“The elution volume is low enough to concentrate any sample very well and the efficiency is not compromised at all.”
– Shanshan L. (UT Southwestern)
“I had low-volume, low-concentration, high-molecular weight genomic DNAs for high-throughput sequencing that I could not get to be perfectly clean without losing a significant amount of my (precious) sample. The Qiagen products were recommended by the company doing the sequencing, and they just ate up my sample. When I received this sample with my mini-prep kit, I was at the end of the line and figured I’d give it a try, despite being warned against anything involving spin columns that may shear the DNA. It worked beautifully. We can proceed with our project. No loss of sample, the gel looks the same (no sign of DNA shearing), and what an easy and fast protocol! Thank you, Zymo!”
– Cindy T. (University of Iowa)
“I sampled this kit next to a kit we already use. I had three of the same sample, two for the Zymo kit and one for the other. On one of the ones designated for your kit, I accidentally added the sample directly to the column filter, then added other reagents (I figured I might as well try it). The other I did as per the instructions. The one on which I goofed gave a similar purification and concentration as the other kit, while the one I did properly yielded approximately four times the DNA per µL as the other kit. I am very happy with this result, and when I rerun my samples, I plan to use your product.”
– Rosalind P. (University of Arkansas for Medical Sciences)
“I like the enhanced design of the column as it funnels every drop of volume directly through the silica in a very efficient manner. The quick spin times and relative ease of use are also a big draw as it cuts down on processing time and allows the attack of subsequent enzymatic steps at a heightened pace.”
– Joseph R. (Miller School of Medicine, University of Miami)
“It was a very simple procedure and it gave a concentration ten times the original amount.”
– Kimberly M. (University of Pennsylvania)
“It only took 2 minutes for the total procedure – shorter than my current method of PCR purification.”
– Marissa V. (Harvard Medical School)
“Almost no loss of elution buffer at the final step. It is quite impressive compared to the other kits I have used, which lose about 5-7µl.”
– Takashi K. (Monash University)
“The simple steps and washing instructions were excellent. DNA was ready for use for PCR and gave strong results.”
– Tyler C.
“This kit performed as described. I was able to obtain good quality and yield of the DNA -better than with a couple of competitor’s kits.”
– Kathleen B. (SUNY-ESF)
DNA Clean & Concentrator-5