Highlights
- Purify cellular and/or cell-free DNA easily and reliably from up to 40 ml of urine.
- Uniquely formulated urine conditioning reagent allows stabilization of DNA in urine for up to 1 month at ambient temperature.
- Zymo-Spin column technology ensures DNA is ready for all sensitive downstream applications including qPCR, DNA sequencing, arrays, and DNA methylation analysis.
Description
Technical Specifications
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation sequencing. |
|---|---|
| Equipment | Centrifuge, microcentrifuge, and heat block/bath. |
| Processing Volume | ≤ 40 ml |
| Purity | Typical A260/A280 ≥ 1.8 |
| Sample Source | Urine |
| Sample Storage | Eluted DNA should be stored at ≤ -20°C. |
| Size Range | 100 bp – 23 kb |
| Type | Total DNA including cfDNA |
| Yield | Up to 5 µg total DNA can be eluted into ≥ 10 µl. 6 – 1000 ng/ml for healthy females. 2 – 20 ng/ml for healthy males. |
Product FAQ
Q1: If I plan to process my urine samples immediately, is the Urine Conditioning Buffer necessary?
The Urine Conditioning Buffer (UCB) is necessary if you are interested in isolating cell-free DNA. If omitting the UCB, only cellular DNA is recovered.
Q2: What is the function of the Clearing Beads?
When the Urine Conditioning Buffer is added to the urine, the Clearing Beads helps to create a compact pellet and make it the pellet easy to visualize during the procedure.
Q3: Can I process urine samples stabilized in DNA/RNA Shield?
Yes, however, the volume of sample processed becomes large and difficult to handle. In addition, cellular and cell-free DNA cannot be separated. To process urine samples stabilized in DNA/RNA Shield, please contact technical support at tech@zymoresearch.com.
Q4: What should I do if I see an oil layer on top of the pellet, after centrifugation?
Remove the oil layer by pipetting and process the pellet. However, residual oil will not affect purification.
Q5: Can cell-free DNA be isolated from urine stored at – 80 °C?
We recommend stabilizing fresh urine with the Urine Conditioning Buffer and storing stabilized urine for later processing. Frozen urine can be processed, however, cells may lyse open upon freeze-thaw, causing in fragmentation of gDNA and resulting in more cell-free DNA than what was originally present.
Q6: Is the Quick-DNA Urine kit compatible with DNA Urine Preserve from Streck?
Yes, however, we cannot guarantee the efficiency of the Streck preservative.
Citations
Reviews
Good yields of DNA in a very simple efficient process.
– R. W. (Medical University of South Carolina)
The kit is very useful as it lets us to analyze big volumes without an ultracentrifugation step.
– S. B.M. (University of Barcelona)
The protocol was easy to use/follow and it didn’t take a great deal of time to get results.
– S. H. (South College School of Pharmacy)
We tested it this week and it worked beautifully. We really would like to order this kit to process more samples!
– R. D. (Medical College of Wisconsin)
The Zymo Kit and more specifically the Cell-DNA method presents as the best option to improve the outcome of our LR-PCR method.
– R. D. (University of Sydney and Royal North Shore Hospital)
Urine DNA isolation kit is very much needed and I like Zymo kits for the simplicity an reliability.
– A. C. (Sanford-Burnham Medical Research Institute)
Kit Components
| Cat # | Name | Size |
|---|---|---|
|
D3004-4-4 |
DNA Elution Buffer | 4 ml |
|
D3004-1-50 |
Genomic Lysis Buffer | 50 ml |
|
D3061-1-140 |
Urine Conditioning Buffer | 140 ml |
|
D3061-2-1 |
Clearing Beads | 1 ml |
|
D3061-3-20 |
Urine Pellet Digestion Buffer | 20 ml |
|
D3061-4-10 |
Urine DNA Prep Buffer | 10 ml |
|
C1001-50 |
Collection Tubes | 50 Pack |
|
C1015-50 |
Zymo-Spin IC-S Columns | 50 Pack |
|
D3061-5-12 |
Urine DNA Wash Buffer | 12 ml |
Quick-DNA Urine Kit
D3061
| Cat # | Name | Size |
|---|
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