The DNA Clean & Concentrator-25 (DCC-25) is a PCR purification kit designed for rapid desalting and purification of up to 25 µg DNA from enzymatic reactions (e.g., PCR), endonuclease digestions, or cell-free lysates. Simply add the specially formulated DNA Binding Buffer to your sample and transfer to the supplied Zymo-Spin column. This PCR purification kit features Zymo-Spin column technology to yield high-quality, purified DNA in just minutes, and it is compatible with cDNA and ssDNA. Eluted DNA is suitable for sequencing, microarray analysis, PCR, nucleotide blotting, and restriction endonuclease digestion procedures.

Detergent Tolerance	≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS
Elution Volume	≥ 25 µl
Equipment	Microcentrifuge
Purity	Highly-pure DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions.
Sample Source	DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc.
Size Range	50 bp to 23 kb
Yield	≤ 25 µg total DNA can be recovered. For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%.

Product FAQ

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“This kit performed as described. I was able to obtain good quality and yield of the DNA -better than with a couple of competitor’s kits.”

– Kathleen B. (SUNY-ESF)

“The simple steps and washing instructions were excellent. DNA was ready for use for PCR and gave strong results.”

– Tyler C.

“It only took 2 minutes for the total procedure – shorter than my current method of PCR purification.”

– Marissa V. (Harvard Medical School)

“Almost no loss of elution buffer at the final step. It is quite impressive compared to the other kits I have used, which lose about 5-7µl.”

– Takashi K. (Monash University)

“I like the enhanced design of the column as it funnels every drop of volume directly through the silica in a very efficient manner. The quick spin times and relative ease of use are also a big draw as it cuts down on processing time and allows the attack of subsequent enzymatic steps at a heightened pace.”

– Joseph R. (Miller School of Medicine, University of Miami)

“I sampled this kit next to a kit we already use. I had three of the same sample, two for the Zymo kit and one for the other. On one of the ones designated for your kit, I accidentally added the sample directly to the column filter, then added other reagents (I figured I might as well try it). The other I did as per the instructions. The one on which I goofed gave a similar purification and concentration as the other kit, while the one I did properly yielded approximately four times the DNA per µL as the other kit. I am very happy with this result, and when I rerun my samples, I plan to use your product.”

– Rosalind P. (University of Arkansas for Medical Sciences)

“The elution volume is low enough to concentrate any sample very well and the efficiency is not compromised at all.”

– Shanshan L. (UT Southwestern)

“It was a very simple procedure and it gave a concentration ten times the original amount.”

– Kimberly M. (University of Pennsylvania)