Anti-5-Hydroxymethylcytosine Polyclonal Antibody

Highlights

Low cross-reactivity with cytosine and 5-methylcytosine versus other available antibodies.
Can be used with a variety of genomic DNA sources.
High sensitivity to low masses of 5-hydroxymethylcytosine DNA.

Description

The rabbit Anti-5-hmC Polyclonal Antibody can robustly distinguish between hydroxymethylated DNA and methylated or unmodified DNA with limited to no cross-reactivity. The antibody has been validated in ELISA and immunoprecipitation-based enrichment assays, and is suitable for use in further applications including immunohistochemical labeling and chromatographic blotting.

Technical Specifications

Buffer Solution	Antibody is provided in PBS (Phosphate Buffered Saline, pH 7.4) with 0.05% Sodium azide as a preservative.
Isotype	IgG1
Protein Concentration	1 mg/ml
Source	Rabbit
Specificity	5-hydroxymethylcytosine in single-stranded DNA from any mammalian, plant, insect, or microbial sources, as well as artificial templates used for standardization.
Storage	Store at -20°C with prolonged storage at -80° C. Avoid repeated freeze/thawing.

Citations

Anti-5-Hydroxymethylcytosine Polyclonal Antibody

A4001-200 / A4001-25 / A4001-50

Cat #	Name	Size
A4001-200	Anti-5-Hydroxymethylcytosine Polyclonal Antibody	200 µg
A4001-25	Anti-5-Hydroxymethylcytosine Polyclonal Antibody	25 µg
A4001-50	Anti-5-Hydroxymethylcytosine Polyclonal Antibody	50 µg

Documents

Protocol:

SDS (MSDS):